I’ll explain how to is possible read the files from Amazon S3 Storage but without using a EC2 instance.
For example your “localhost” or your Standalone Wowza server running on your own datacenter.

I did this for working on my local development environment, I’ll describe the steps for a Linux environment.

1- Download from this URL the 2 Jars, and copy this in /lib directories.

wms-plugin-amazonaws.jar (1109)
wms-plugin-mediacache.jar (1111)

2- Create the MediaCache folder:

mkdir /mnt/mediacache

3- Insert into the conf/Server.xml this lines:

<ServerListeners>
      <ServerListener>
                <BaseClass>com.wowza.wms.plugin.amazonaws.ec2.mediacache.MediaCacheServerListenerAmazonEC2</BaseClass>
       </ServerListener>
</ServerListeners>

4- Insert into the conf/vods3/Application.xml (create this first) this lines:

                <MediaReader>
                        <Properties>
                                <Property>
                                        <Name>randomAccessReaderClass</Name>
                                        <Value>com.wowza.wms.plugin.amazonaws.ec2.mediacache.MediaCacheRandomAccessReaderAmazonEC2</Value>
                                </Property>
                                <Property>
                                        <Name>bufferSeekIO</Name>
                                        <Value>true</Value>
                                        <Type>Boolean</Type>
                                </Property>
                        </Properties>
                </MediaReader>

And in the end of the file, add this properties (I don’t know is needed this for read Public content):

                <Properties>
                        <!-- Set these two properties to do S3 authentication -->
                        <Property>
                                <Name>awsAccessKeyId</Name>
                                <Value>KEY</Value>
                        </Property>
                        <Property>
                                <Name>awsSecretAccessKey</Name>
                                <Value>KEY</Value>
                        </Property>
                </Properties>

5- Restart the Wowza Server and try reading from S3 Bucket.

The URL can be like this:
rtmp://localhost/vods3/_definst_/mp4:amazons3/wmconsulting.content/Extremists.m4v

Tell me is I missing some step or need some extra help.